Microneedle Skin Patch Collects Fluid for Diagnostic Testing

A new study describes how a paper-based skin patch that contains tiny needles painlessly collects interstitial fluid (ISF) for biomarker analysis.

Developed at Washington University (WUSTL; St. Louis, MO, USA) and the Georgia Institute of Technology (Georgia Tech; Atlanta, GA, USA), the patch’s micrometer-scale microneedles puncture the skin, creating tiny micropores in the surface through which small quantities of ISF leak. The ISF is subsequently collected onto a strip of plasmonic paper that forms part of the backing of the patch. It is then analyzed in-situ, using surface-enhanced Raman scattering (SERS), in order to detect and measure pharmacokinetic profiles.

For the study, the researchers immobilized negatively charged poly(styrenesulfonate) (PSS) coated gold nanorods on a thin strip of filter paper using plasmonic calligraphy. A positively charged dye containing rhodamine 6G (R6G) was then injected into a rats' bloodstream. The dye entered the ISF and from there to the microneedle patch and onto the plasmonic paper, where it bound to the negatively charged PSS, and then analyzed with SERS. The researchers found that the new method could detect the R6G dye as sensitively as previous multi-step procedures. The study was published on May 9, 2019, in ACS Sensors.

“This proof-of-concept study indicates that a plasmonic paper microneedle patch has the potential to enable on-patch measurement of molecules in ISF for research and future medical applications,” concluded lead author Chandana Kolluru, PhD, of the Georgia Tech School of Materials Science and Engineering, and colleagues.

ISF surrounds all tissue cells and is present in the skin. It is advantageous for biosensing applications since it does not contain any particulates (red blood cells or platelets), and contains at least 5–10 times less protein than blood serum. However, only extremely low volumes can be found on the skin, making the process of ISF extraction rather difficult. ISF extraction using microneedles combined with integrated biosensing capabilities could provide significant opportunities for minimally invasive monitoring and diagnostics, such as in diabetes.

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Georgia Institute of Technology