SPECT/CT Plus Enzyme Found to Be Best Imaging Modality for Detecting Coronary Arterial Disease

The key role a single enzyme plays in cancer imaging had confounded researchers for years; however, this new finding could provide new applications in nuclear medicine. The enzyme, called neutral endopeptidase (NEP), can degrade most radiopeptide imaging agents in the body. Researchers have developed a well-designed new hypothesis that improves molecular imaging, according to their findings, which were presented during the Society of Nuclear Medicine and Molecular Imaging’s 2014 annual meeting, held June 7-11, 2014, in St. Louis (MO, USA).

The enzyme has dodged studies with peptide tracers until now because it lives not in tested blood serum but along the walls of blood vessels and other tissues. To counteract the degradation of circulating radiopeptides, researchers co-injected a NEP inhibitor called phosphoramidon, derived from bacteria; at the same time as an agent for imaging with single photon emission computed tomography and computed tomography (SPECT/CT). They then applied this method of enzyme inhibition in multiple imaging studies involving a range of radionuclide and peptide counterparts. The results of this research showed consistent success—up to 40 times the circulating radiopeptides when protected with phosphoramidon, compared to unprotected controls. This means the simple co-injection of an enzyme inhibitor promotes dramatically improved bioavailability and metabolic stability of radiopeptide imaging agents leading to higher uptake of the agent within targeted tumors and therefore better cancer imaging.

“Oncologists have long sought a powerful ‘magic bullet’ that can find tumors wherever they hide in the body so that they can be imaged and then destroyed,” said Marion de Jong, PhD, a lead researcher for this study conducted at Erasmus Medical Center (Rotterdam, The Netherlands) in cooperation with the National Center for Scientific Research (NCSR) Demokritos (Athens, Greece). “Following this innovative approach, we have been able to induce, for the first time, an impressive improvement in the level of circulating and viable radiopeptides, leading to a spectacular increase in tumor uptake. Enzyme-inhibition in the body could translate into higher diagnostic sensitivity and improved therapeutic efficacy of radiopeptide drugs in cancer patients.”

Not only were circulating radiopeptides increased in small animal models of varying tumor types, but the accumulation of radiopeptides also peaked at 14 times that of controls, which had not been treated with enzyme-inhibiting phosphoramidon. These findings were clearly visualized by SPECT/CT imaging.

Related Links:
Erasmus Medical Center
National Centre for Scientific Research Demokritos