New Assay Detects Brazilian and South African SARS-CoV-2 Variants Faster than Sequencing, Screens More Samples

The assay uses common qPCR instruments with detection channels of FAM, HEX, Cy5, and ROX. Using RT-qPCR to detect the SARS-CoV-2 E484K variant is faster and allows far more samples to be screened, compared to sequencing. The AzureSeq E484K assay quickly identifies a mutation found in SARS-CoV-2 variants SA B.135.1 and Brazil P.1, which have been labeled as "variants of concern" by the CDC due to the potential to impact vaccine efficacy, and which may also be more infectious. The AzureSeq One-Step Universal SARS-CoV-2 RT-qPCR E484K Assay is currently for Research Use Only.

"The release of the AzureSeq E484K assay is an important addition to our recently launched AzureSeq N501Y assay. While sequencing is the gold standard, using the AzureSeq RT-qPCR assays will screen far more samples for these Variants of Concern, and will do it in less than an hour - compared to two days," said Joe Dunham, CSO of SeqOnce.

"We anticipate teams using the AzureSeq assays to screen for these Variants of Concern will have reduced barriers. The assays work on conventional qPCR instruments that are wide-spread, and do not require specialized knowledge in sequencing," added Chris Angermayer, CEO of SeqOnce Biosciences. "Basically, if a lab can run a qPCR SARS-CoV-2 assay, they can also now screen for these variants."

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