Biocompatible Scaffolds Made From Unique Hybrid Materials Can Repair Spinal Cord Tissue

The debilitating disorder for which there is currently no widely available treatment results in paralysis below the level of injury. In the US alone, the annual healthcare costs for spinal cord injury patient care stand at USD 9.7 billion. Now, a team of scientists has developed a unique new material that has shown significant promise in the treatment of spinal cord injury.

Scientists at the University of Limerick (Limerick, Ireland) have developed new hybrid biomaterials in the form of nanoparticles that were successfully synthesized to promote repair and regeneration following spinal cord injury. The team used a new kind of scaffolding material and a unique new electrically conducting polymer composite to promote new tissue growth and generation that could advance the treatment of spinal cord injury. There has been growing interest in the use of electroconductive tissue engineered scaffolds due to the improved cell growth and proliferation when cells are exposed to a conductive scaffold.

“Raising the conductivity of biomaterials to develop such treatment strategies typically centres on the addition of conductive components such as carbon nanotubes or conductive polymers such as PEDOT:PSS, which is a commercially available conductive polymer that has been used to date in the tissue engineering field,” explained lead author Aleksandra Serafin, a PhD candidate in the Bernal and at UL’s Faculty of Science and Engineering. “Unfortunately, severe limitations persist when using the PEDOT:PSS polymer in biomedical applications. The polymer relies on the PSS component to allow it to be water soluble, but when this material is implanted in the body, it displays poor biocompatibility.

“This means that upon exposure to this polymer, the body has potential toxic or immunological responses, which are not ideal in an already damaged tissue which we are trying to regenerate. This severely limits which hydrogel components can be successfully incorporated to create conductive scaffolds,” added Serafin.

Novel PEDOT nanoparticles (NPs) were developed in the study to overcome this limitation. Synthesis of conductive PEDOT NPs allows for the tailored modification of the surface of the NPs to achieve desired cell response and increasing the variability of which hydrogel components can be incorporated, without the required presence of PSS for water solubility. In this work, hybrid biomaterials comprised of gelatin and immunomodulatory hyaluronic acid, was combined with the developed novel PEDOT NPs to create biocompatible electroconductive scaffolds for targeted spinal cord injury repair.

A complete study of the structure, property, and function relationships of these precisely designed scaffolds for optimized performance at the site of injury was carried out, including in-vivo research with rat spinal cord injury models. Biological response to the developed PEDOT NP scaffolds was studied with stem cells in-vitro and in animal models of spinal cord injury in-vivo. Excellent stem cell attachment and growth on the scaffolds was observed, they reported. Testing showed greater axonal cell migration towards the site of spinal cord injury, into which the PEDOT NP scaffold was implanted, as well as lower levels of scarring and inflammation than in the injury model which had no scaffold, according to the study. Overall, these results show the potential of these materials for spinal cord repair, say the research team.

“The impact that spinal cord injury has a on a patient’s life is not only physical, but also psychological, since it can severely affect the patient’s mental health, resulting in increased incidences of depression, stress, or anxiety,” explained Ms. Serafin. “Treating spinal injuries will therefore not only allow for the patient to walk or move again but will allow them to live their lives to their full potential, which makes projects such as this one so vital to the research and medical communities. In addition, the overall societal impact in providing an effective treatment to spinal cord injuries will lead to a reduction in health care costs associated with treating patients. These results offer encouraging prospects for patients and further research into this area is planned.”

“Studies have shown that the excitability threshold of motor neurons on the distal end of a spinal cord injury tends to be higher. A future project will further improve the scaffold design and create conductivity gradients in the scaffold, with the conductivity increasing towards the distal end of the lesion to further stimulate neurons to regenerate,” she added.

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University of Limerick