We use cookies to understand how you use our site and to improve your experience. This includes personalizing content and advertising. To learn more, click here. By continuing to use our site, you accept our use of cookies. Cookie Policy.

Features Partner Sites Information LinkXpress
Sign In
Advertise with Us
Detecto

Download Mobile App





Delivering COVID-19 Vaccines via Suctioning Technique May Help Generate Higher Antibody Levels

By HospiMedica International staff writers
Posted on 08 Nov 2021
Print article
Image: Portable, Battery-Powered Suction Device (Photo courtesy of Rutgers University)
Image: Portable, Battery-Powered Suction Device (Photo courtesy of Rutgers University)

Researchers studying COVID-19 have created a new way to deliver DNA molecules into skin cells, using a suction technique similar to the ancient healing practice of cupping.

In laboratory tests with rodents, the team of researchers at Rutgers University (New Brunswick, NJ, USA) used the suction method to deliver a SARS-CoV-2 DNA vaccine, which generated a strong immune response – about 100 times stronger than an injected vaccine alone. Cupping is a traditional practice in which heated cups are placed on the skin to create negative pressure, increasing blood circulation to the area in an effort to promote healing. Nucleic-acid medicine is a next-generation technology using DNA, RNA and other biomolecules that control genetic information. It has grown extensively over the past two decades due to its promise in treatments and vaccines for various diseases. Most recently, several nucleic-acid-based vaccines have been rapidly designed, manufactured and mass-distributed to fight the COVID pandemic.

Nucleic-acid medicine works when synthetic or engineered nucleic acids enter host cells and, using the cellular machinery, direct the production of encoded proteins to prompt an immune response in case of a vaccine. A key step in this process is transfection, or the delivery of purified nucleic acids across the cell-membrane barriers into the cytoplasm (RNA) and nucleus (DNA) of host cells. If DNA and RNA molecules are injected into tissue, they do not automatically enter the host cells and most will rapidly degrade unless they are protected.

For example, in mRNA-based COVID vaccines, lipid nanoparticles are used to enclose the mRNAs to protect and help deliver them across the host cell membrane, so that the coded protein is produced and an immune response provoked. Alternately, an electrical field is often used to deliver DNA to cells, but this method commonly causes adverse effects, including inflammation, pain and tissue damage. However, in the new study, after the injection of pure DNA, the researchers applied suction directly to the site to create negative pressure atop the skin. The suction produced strain and relaxation in the layers of skin, triggering uptake of the DNA molecules by skin cells. The new method is simple, painless and has no known side effects, according to the researchers.

“This suction-based technique is implemented by applying a moderate negative pressure to the skin after nucleic acid injection in a totally non-invasive manner,” said the study’s senior author Hao Lin, a professor in the Department of Mechanical and Aerospace Engineering at Rutgers-New Brunswick. “This method enables an easy-to-use, cost-effective and highly-scalable platform for both laboratory and clinical applications for nucleic-acid-based therapeutics and vaccines.”

“Development of enhanced delivery technologies plays an instrumental role in bringing nucleic-acid based biologics to broad use and clinical relevance, and world-wide vaccine distribution is just one example,” he said. “We have demonstrated an alternative, safe and effective transfection platform that yields high levels of transgene expression. The advantages also include device cost-effectiveness and manufacturing scalability and minimal requirements for user training. Because of the inherent advantages of DNA, not least of which is avoiding cold-chain requirements of other vaccines, this technology facilitates vaccination programs into remote regions of the world where resources are limited.”

Related Links:
Rutgers University 

BMP Whole Blood Analyzer: GEM Premier ChemSTAT
New
Gold Supplier
Real-Time Diagnostics Onscreen Viewer
GEMweb Live
New
Gynecological Chair
FG-07
New
Heating Lamp
Isotherm

Print article

Channels

AI

view channel
Image: EchoGo Heart Failure is the first and only AI-enabled HFpEF detection platform for echocardiography (Photo courtesy of Ultromics)

AI Solution for Echocardiography to Revolutionize Diagnosis of Heart Failure with Preserved Ejection Fraction

Heart failure with preserved ejection fraction (HFpEF) is a type of heart failure often associated with co-morbidities and tends to be caused by increased pressure within the chambers of the heart.... Read more

Critical Care

view channel
Image: Lodestar DX provides lab-accurate test results in 30 minutes (Photo courtesy of Llusern Scientific)

Portable UTI Molecular Diagnostic Platform Provides Accurate Test Results in 40 Minutes

Despite being one of the most common types of infection Urinary Tract Infections (UTIs) are a comparatively neglected area of medicine. There are no highly accurate diagnostic tools available for point-of-care... Read more

Surgical Techniques

view channel
Image: ActivSight Intelligent Light has received CE Mark approval (Photo courtesy of Activ Surgical)

Enhanced Imaging System to Become a Game-Changer in the OR by Revolutionizing Surgical Vision

Critical structure identification and tissue perfusion assessment are essential for patients to have the best possible chance of healing well without facing life-threatening or costly complications.... Read more

Patient Care

view channel
Image: Automated cleaning system allows endoscopes to be cleaned direct from clinic (Photo courtesy of Aston University)

World’s First Automated Endoscope Cleaner Fights Antimicrobial Resistance

Endoscopes are long, thin tubes with a light and camera at one end. Due to the sensitivity of the materials and electronics they cannot be sterilized in an autoclave (a machine that uses steam under pressure),... Read more

Health IT

view channel
Image: Using digital data can improve health outcomes (Photo courtesy of Unsplash)

Electronic Health Records May Be Key to Improving Patient Care, Study Finds

When a patient gets transferred from a hospital to a nearby specialist or rehabilitation facility, it is often difficult for personnel at the new facility to access the patient’s electronic health records... Read more

Business

view channel
Image: The global multiparameter patient monitoring systems market is expected to surpass USD 15 billion by 2028 (Photo courtesy of Unsplash)

Global Multiparameter Patient Monitoring Systems Market Driven by Rising Chronic Illnesses

Multi-parameter patient monitoring equipment is used to assess the vital signs of patients who are suffering from a serious illness. These devices are meant to give the number of data sets on one screen... Read more
Copyright © 2000-2022 Globetech Media. All rights reserved.