Tiny Biosensor for Use in Brain Detects Biomarkers Tied to Traumatic Brain Injuries

The waterproof biosensor developed by researchers at the Ohio State University (Columbus, OH, USA) includes an “unprecedented combination of features” that may allow it to detect changes in the concentrations of various chemicals in the body and send the results to researchers in real time. The chip is flexible and thinner than a human hair, making it minimally invasive for use in the brain.

Although a biosensor such as the one the team developed could have many potential uses, the researchers were particularly focused in this study on how the sensor could be used to monitor patients with TBI. After such an injury, secondary damage can occur that can be detected by changes in sodium and potassium ion concentrations in the brain’s cerebrospinal fluid. The researchers tested the biosensor in an artificial solution they created to mimic cerebrospinal fluid and found that it could accurately detect changes in potassium and sodium ion levels that are important in TBI. In addition to the tests with the artificial cerebrospinal fluid, the team also tested the biosensor in human blood serum, in which they successfully monitored pH levels.

The chip features electronic components (known as field-effect transistors) that, upon sensing the chemical of interest, produce an electrical signal that can be detected and analyzed outside the body. Importantly, the researchers developed calibration standards that address what is called the “crosstalk” issue. While a biosensor has to be able to detect changes in the fluids in the brain, the electronics in the chip must be protected from these same fluids. A waterproof encapsulation made from a thin film of silicon dioxide – forged in temperatures above 1,000 degrees Celsius – provided high structural integrity as barrier materials in a fluid environment, the study found.

In order to find out how long the encapsulation could last in the human body, the researchers tested the material in a variety of ways, such as by placing it in heated fluids and in substances with different pH levels. Their findings suggest that waterproof encapsulation with a thickness of several hundreds of nanometers could last at least a few years at body temperature and possibly much longer. The biggest issue right now is with the chemical sensing elements, which the study suggests would work for only up to a few weeks. There are also other issues that need to be worked out before the biosensor is ready to be tested in animal models and humans. The response of biotissues to the sensor over extended period needs further study. There are still issues with crosstalk to be resolved, considering the complexity of the biosystem, and questions of how to mass produce the sensors, among other matters.

However, the study provides more evidence that these sensors have a real future in health care. The researchers believe that biosensors could be used to analyze not only ions and neurotransmitters, as in this study, but possibly peptides, proteins, nucleotide acids and other chemicals in the body. It could be a breakthrough not only for TBI, but for other chronic diseases such as Parkinson’s and Alzheimer’s disease.

“We have a long way to go from our tests in the lab, but these findings were very encouraging,” said study co-author Jinghua Li, assistant professor of materials science and engineering at Ohio State. “We believe that the capture and analysis of health data that we could achieve with biosensors are crucial to tracking long-lasting health conditions for early intervention and treatment of diseases.”

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